AnyDetect
AnyDetect
Portable Real-Time
Isothermal Amplifier
With the development of AnyDetect, a genetic test that used to take several days for a definitive diagnosis by sending it to a specialized laboratory, even unskilled workers can easily test it on the spot.
DNA/RNA isothermal amplification device employing LAMP method
Any Detect is a general-purpose equipment that can apply all isothermal amplification technologies such as LAMP and RPA, and it is equipped with an amplification reaction unit and a detection unit to interpret the results in real time.
LAMP
Steps of temperature adjustment
isothermal amplification
（Single Step）
PCR
Complicated
(Thermal cycling)
Detection Speed
〜30min.
〜120min.
Sensitivity to Specimen Inhibitors
Less effect of inhibitors
susceptible to the influence of inhibitors
Necessity of amplification confirmation device
Not required
(check with Real Time)
Required dyeing reagent or
gel electrophoresis
Battery-powered portable detection device
It is equipped with a rechargeable battery (5,200mAh), so it can operate for more than 6 hours on a single charge.
6 hours
Compact design
Compact design optimized for ultra light weight (approx. 850 g)
Simultaneous detection of 8 channels
8 specimens can be set, allowing simultaneous testing of 8 samples.
High on-site availability
As it is powered by a rechargeable battery, it has portability and fieldability, so it can be expected to quickly respond to various situations such as livestock farms, food processing sites, and public facilities and environmental pollution investigations.
노로바이러스
장관출혈성대장균
(O-157)
캠필로박터
살모넬라
조류독감
Detection example
Enterohemorrhagic E. coli O-157
Positive
Negative
Shows the results of testing positive samples and negative controls with our LAMP kit that can detect O157 E. coli. In the positive sample, an amplification signal appeared from around 23 minutes (red arrow), whereas in the negative control group, no amplification signal was formed until the reaction was completed (blue arrow).
Campylobacter
Our LAMP kit test results are shown for standard samples serially diluted with Campylobacter bacteria. For 1,000 copies, the amplification signal appears from about 25 minutes after the start of the reaction, for 500 copies, around 32 minutes, and for 100 copies, the amplification signal appears around 39 minutes. In particular, it can be seen that the amplification signal is confirmed around 45 minutes even when reacting with a trace sample of 10 copies.
1,000 copy
500 copy
100 copy
10 copy
Interlocking with PC and smartphone
Can check detailed measurement results in real time with a PC program and a smartphone app.
Micro 5pin
Smartphone
(IOS / Android)
PC
(Windows 8/10)
FAQ
Q. What kind of amplification technology can AnyDetect apply to?
A. Any isothermal amplification method that reacts at a temperature within the operating range of 40-80 degrees is applicable. In particular, we are providing various test kits for the LAMP test method.
Q. How should the sample be prepared?
A. Compared to other test methods, our LAMP kit is more tolerant of inhibitors and can be applied without purification. However, to ensure high specificity and sensitivity, it is recommended to use DNA/RNA separated/purified by column or bead method.
Q. How long does detection take?
The time taken for the amplification reaction can be fast or slow because it is affected by various factors such as the amount of the sample, the purity of the sample, and the external environment. The LAMP kits we provide are optimized to confirm results, typically within 10 to 40 minutes.
Q. How can the positive result of the target be confirmed?
When an amplification reaction occurs in a positive sample through our LAMP kit, the degree of turbidity becomes severe, and the presence or absence of positive/negative is checked through the detection unit of AnyDetect, which can detect turbidity in real time.
Q. What is the difference between PCR test and LAMP test?
PCR requires thermal cycling, whereas LAMP reacts at a single temperature, allowing for miniaturization of the device and good portability. While PCR increases amplification products by a factor of two for each reaction cycle, LAMP produces more amplification products in the same time as there is no limit to this. In the case of the actual LAMP test, it is often possible to determine the result within 15 minutes. In addition, the LAMP test method has the advantage of being less restrictive in sample preparation because it is more tolerant of reaction inhibitors than PCR.
Specification
Item
Contents
Test Wells
200㎕ 8 tubes PCR Stripe
Optical Wavelength
650nm
Reaction Control Temp.
50℃ ~ 80℃
Temp. Difference
±0.5℃
Scan Interval
10 second
Interface
Bluetooth (Mobile Phone : Android, iOS)
Micro USB 5pin (USB Type A PC/Notebook)
Display
LCD 4.3“
Storage
SD Card
Power
AC/DC Adapter(Charger) : 100~240Vac 50/60Hz, 9Vdc/3A
Battery Life : Min. 6 hours @60℃ vs 25℃ ambient
Dimension
150mm x 169mm x 89mm
Weight
~900g
Operating Environment
Temperature: 10℃ ~ 40℃ / Humidity: 10% ~ 80%
Storage Environment
Temperature: 5℃ ~ 50℃ / Humidity: 10% ~ 80%